The quality of images captured from your microscope can be negatively affected by uneven lighting conditions or artifacts introduced into optical path. Besides creating obvious aesthetic problems, these conditions also can create inaccurate image analysis data because detected objects or thresholded pixels may be unintentionally included. Fortunately, PAXcam offers a quick and easy solution for you: real time background subtraction.
Background subtraction is a method for correcting images for uneven illumination and for items in the final image that are not truly part of the subject matter. It involves taking two images: one with the sample in place and a control image with no sample in place, and subtracting the control image from the sample image. The resulting image is more visually pleasing and is more suitable and accurate for use in image analysis routines. Obviously, it is best to correct the uneven illumination or remove the artifacts prior to capturing your image, as it results in an image with unaltered native data. However, if this is not an option, the real time background subtraction feature available with a PAXcam digital microscope camera offers the next best alternative.
PAXcam’s background correction can be used to correct the live image from the camera in one of two ways depending on your subject matter
1. Subtraction. This method subtracts the background image from the live image, thereby correcting for specific points in the image that are brighter or darker than the general background.
2. Division. This method divides the live image by the background image at each pixel site in the image
Image before background subtraction.
Image after background subtraction.
When you capture the image, the image that appears in your PAX-it workspace will automatically have the background corrector image applied to it. Making your images look great by using PAXcam is easy!