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Sperm Analysis

 

Accurately processing rape kits for sperm identification in the traditional manner can be quite time consuming.  Sperm is often indistinguishable or difficult to identify relative to the rest of the sample, especially when being viewed through a brightfield microscope.  These manual scans of slides can result in user fatigue and the potential for missing positive samples.

Brightfield Sperm Analysis


However, the PAXcam Microscope Camera system, used in conjunction with new revolutionary staining techniques for fluorescence microscopy, makes sperm identification and documentation quick, accurate and easy.  The dual staining technique uses one stain for cell nuclei in general, and another that is specific for human sperm. 

Sperm Analysis


When switching to the sperm-specific stain under fluorescence microscopy, human sperm heads are clearly identified and countable, standing out from the background material due to their unique staining properties.

Sperm Analysis


Brightfield and fluorescent images can be captured from the PAXcam and stored in the PAX-it image database software, where they can be labeled and identified by case number and other user-defined tags, for easy retrieval in the future.   
Once the images have been stored, the PAX-it image analysis software can easily count the number of sperm in the field, creating repeatable processes, eliminating human error, and greatly reducing processing and documentation times.  That means many more cases can be analyzed in less time with more accuracy.  A winning combination!

Sperm Analysis


Image blending techniques in the PAX-it software allow fluorescent and brightfield images to be overlaid on each other, to show specifically in the field where the human sperm can be found.

Human Sperm Identification


Forensic reports
can be created with the click of a button.  Simply select the icon of the saved image from the PAX-it database, select one of the user-created MS Word, Excel or PowerPoint templates of your choice and PAX-it automatically assembles the customized report in seconds.  Your forensic reports can include images, annotations, notes, database information, measurement and image analysis data, graphs……anything that you need.
All of your images, reports and analysis results can be further secured using PAX-it’s optional Extended Security Module, which enables the user to apply verifiable digital signatures to images and maintain the integrity of data through audit trails automatically created within PAX-it.

Forensic reports can be created by simply selecting the images you wish to put into the report, and then selecting the user-designed report template.  PAX-it combines the images, database information and analysis results into the report automatically.

Automated Sperm Analysis

 

 

 

If rape kits are being processed in a batch mode, users may automate the process by using the PAX-it motorized stage control.  This optional module will allow multiple samples to be placed on the microscope stage, where they can be scanned, imaged, counted, and reported automatically.  The tedious manual scan for sperm is no longer necessary.  Detailed scripts created by the user control the camera, motorized stage (retrofittable on most microscopes) and analysis functions so that multiple cases can be handled with “one click” ease.

 

 

 

 
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Learn more about the PAXcam Digital Microscope Camera and the PAXcam Telepathology module
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